(Phospho-S2054) Rabbit Polyclonal Antibody (APC)

SKU: orb213563

Description

The APC (Phospho-S2054) Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S2054 of human APC protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Cancer, Epigenetics, Neuroscience, Signaling Pathways, Wnt

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1-500-1-1000
• Reactivity: Human, Monkey, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S2054 of human APC protein. The exact sequence is proprietary.
• Target: APC
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5

Western blot analysis of APC (Phospho-S2054) expression in HeLa (A), Caco2 (B), SW480 (C), MCF7 (D), SP2/0 (E), PC12 (F) whole cell lysates. (Predicted band size: 311 kD; Observed band size: 310 kD)

Immunohistochemical analysis of APC (Phospho-S2054) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

APC

UniProt

P25054, P70478, Q61315

NCBI Gene ID

324, 24205