APC Rabbit Monoclonal Antibody (APC)

SKU: orb546977

Description

APC Rabbit Monoclonal Antibody (APC)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Disease Biomarkers, Epigenetics & Chromatin, Neuroscience, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution Range: WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 IP 1:20
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: FAD-1
• Immunogen: A synthesized peptide derived from human APC Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF) -induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.
• Target: Adenomatous polyposis coli protein
• Molecular Weight: 160 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3; APRF

WB analysis of APC using anti-APC antibody in 293T Cell.

IHC analysis of APC using anti-APC antibody in human colon cancer samples.APC was detected in paraffin-embedded section.

Quick Database Links

Gene Symbol

Adenomatous polyposis coli protein

UniProt

P25054