Anti-AMPK gamma 1/2/3 Antibody

• Catalog Number: orb515562
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PRKAG1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AMPK gamma 1/2/3. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PRKAG1; PRKAG2; PRKAG3
• Entrez: 19082, 108099, 5571, 51422, 241113, 25520, 53632
• UniProt ID: Q9UGJ0, Q91WG5, Q9UGI9, O54950, P80385, P54619, Q8BGM7
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: PRKAG1; 5'-AMP-activated protein kinase subunit ga
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AMPK gamma 1/2/3 expression in HEK293T (A), HepG2 (B), MCF7 (C), PC3 (D) whole cell lysates. (Predicted band size: 37; 63; 54 kD; Observed band size: 38 kD)

Immunofluorescent analysis of AMPK gamma 1/2/3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.