AMPK beta 1 Mouse Monoclonal Antibody

SKU: orb1474320

Description

The AMPK beta 1 Antibody is suitable for IF, IHC, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant human AMPK beta 1 protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Dilution Range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant human AMPK beta 1 protein fragments expressed in E.coli.
• Target: PRKAB1
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AMPK; 5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb

Western blot analysis of AMPK beta 1 expression in NIH3T3 (A), Hela (B), PC12 (C), COS7 (D), MDAMB468 (E) whole cell lysates. (Predicted band size: 30 kD; Observed band size: 38 kD)

Immunohistochemical analysis of AMPK beta 1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.33). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of AMPK beta 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

PRKAB1

UniProt

Q9Y478, P80386, Q9R078

NCBI Gene ID

5564, 19079, 83803