AMPK alpha 1/2 (Phospho-T183/172) Antibody

• Catalog Number: orb224175
• Category: Antibodies
• Description: The AMPK alpha 1/2 (Phospho-T183/172) Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T183/172 of human AMPK alpha 1/2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Gallus, Human, Mouse, Porcine, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T183/172 of human AMPK alpha 1/2 protein. The exact sequence is proprietary.
• UniProt ID: P54646, P54645, Q13131, Q8BRK8, Q5EG47, Q09137
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200, IP: 1:10-1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PRKAA1; AMPK1; 5'-AMP-activated protein kinase cat
• Research Area: Autophagic, Cancer, Epigenetics, Neuroscience, Sig
• Note: For research use only
• Entrez: 5563, 5562, 108079, 65248, 78975, 105787
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AMPK alpha 1/2 (Phospho-T183/172) expression in Jurkat Adriamycin-treated (A), K562 Adriamycin-treated (B) whole cell lysates. (Predicted band size: 64; 62 kD; Observed band size: 63 kD)

Immunohistochemical analysis of AMPK alpha 1/2 (Phospho-T183/172) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Direct ELISA antibody dose-response curve using Anti-AMPK alpha 1/2 (Phospho-T183/172) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.