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Catalog Number | orb412026 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to BIN1 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human, Rat |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Immunogen | Recombinant fusion protein of human Amphiphysin 2 |
UniProt ID | O00499, O08839 |
Tested applications | IF, IHC, WB |
Dilution range | WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | AMPHL; Myc box-dependent-interacting protein 1; Am Read more... |
Research Area | Developmental Biology, Epigenetics, Infectious Dis Read more... |
Note | For research use only |
Entrez | 117028, 274 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Amphiphysin 2 expression in SW620 (A) whole cell lysates. (Predicted band size: 45-64 kD; Observed band size: 65 kD)
Immunofluorescent analysis of Amphiphysin 2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, IP, WB | |
Human | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Human, Mouse | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |