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alpha/beta-Tubulin dimer Antibody

SKU: orb44536

Description

Mouse Monoclonal to alpha/beta Tubulin dimer.

Images & Validation

Tested ApplicationsICC, WB
ReactivityHuman, Mouse, Porcine
Application Notes
Western blotting: Recommended dilution: 2 μg/ml; positive control: porcine brain lysate.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgM
Clone No.TU-08
ImmunogenMicrotubule proteins from porcine brain.
Targetalpha/beta-Tubulin dimer
PurificationPurified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

TUBA, TUBB

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alpha/beta-Tubulin dimer Antibody

Western blotting analysis of human alpha/beta-tubulin using mouse monoclonal antibody TU-08 on lysates of various cell lines and porcine brain under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-alpha/beta-tubulin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Alpha/beta-tubulin was detected at approximately 54 kDa.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ICC
Immunocytochemistry
View Protocol

alpha/beta-Tubulin dimer Antibody (orb44536)

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0.1 mg
$ 330.00
DispatchUsually dispatched within 5-10 working days.
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