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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 3.513888889, IHC: 1:500-1:1000, ICC: 1:500-1:1000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant construct containing the 7th, 8th, 9th repeats of human alpha II spectrin. |
| Target | Alpha II Spectrin |
| Molecular Weight | 240 |
| Purification | Serum |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Neat serum |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Immunofluorescence of cultured neurons and glia cells showing specific labeling of neuronal perikarya, axons and dendrites with anti-alpha-II-spectrin (Cat. orb319455, 1:1000, green), specific labeling of intermediate filaments in glial and fibroblastic cells with anti-vimentin (Cat. orb319707, 1:5000, red) and nuclear staining with DAPI (blue).

Western blot of mouse brain lysate showing specific immunolabeling of the ~240 kDa alpha II spectrin protein.
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Protocol Information
Alpha II Spectrin Antibody (orb319455)
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