Anti-alpha-gal epitope [m86]

SKU: orb1463377

Description

Mouse monoclonal antibody to alpha-gal epitope

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, FC
• Reactivity: All

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgM
• Clone No.: m86
• Immunogen: Rabbit red cells, on which α-gal is the major carbohydrate epitope, were injected into alpha 1,3 galactosyltransferase knock-out mice.
• Target: alpha-gal epitope
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

A-Gal; Gal-alpha-1 – 3Gal-beta-1 – 4GlcNAc-R

Flow cytometry using the Anti-alpha-gal epitope antibody m86. Paraformaldehyde-fixed RAW264.7 cells were stained with anti-unknown specificity antibody (orb256449; isotype control, black line) or the mouse IgG1 version of m86 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of RAW264.7 cells using anti-alpha-gal epitope antibody m86. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells stained with the chimeric mouse IgG version of m86 at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue).

Western Blot using anti-alpha-gal epitope antibody m86. rat spleen(A) and pig spleen(B) tissue lysate (35 µg protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of m86 at 0.3 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

alpha-gal epitope