Anti-Alpha-fetoprotein (AFP) [AF5]

SKU: orb411562

Description

Mouse monoclonal antibody to AFP

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IF, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: AF5
• Immunogen: AF5 was prepared by immunizing female BALB/c mice with human amniotic fluid containing AFP, from a pregnancy affected by a neural tube defect, diluted and emulsified in Freund's complete adjuvant.
• Target: Alpha-fetoprotein (AFP)
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

A-Feto-protein

Flow-cytometry using the anti-Alpha-fetoprotein AF5 HepG2 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of AF5 (orb411562, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunofluoresence staining of fixed HepG2 cells with anti-Alpha-fetoprotein (AFP) antibody AF5. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of AF5 (orb411562) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb411562, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-Alpha-fetoprotein antibody AF5 HepG2 cell lysate (35 µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of AF5 (orb411562) at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-fetoprotein is 68.7kDa, though this protein is also glycosylated (Uniprot ID: P02771). orb411562 successfully detected human Alpha-fetoprotein in HepG2 cell lysates.

Quick Database Links

Gene Symbol

Alpha-fetoprotein (AFP)

UniProt

P02771