Anti-alpha catenin [1G5]

SKU: orb2645622

Description

Anti-alpha catenin [1G5]

Research Area

Cell Biology, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IF, IP, WB
• Reactivity: Human, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 1G5
• Immunogen: This antibody was raised by immunizing Balb/c mice with a 90 kDa MBP/alpha catenin fusion protein purified on an amylose column.
• Target: alpha catenin
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Catenin alpha-1; Alpha E-catenin; Cadherin-associated protein; Renal carcinoma antigen NY-REN-13; α-catenin; α catenin; α(E)-catenin

Immunofluorescence staining of fixed A431 cells with anti-alpha catenin antibody 1G5. Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 1G5 (orb2645622) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb2645622, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti- alpha catenin antibody 1G5 A431(A) (0.003 µg/ml), HeLa(B) (0.03 ug/ml) and MCF7(C) (0.01 ug/ml) cell lysate. (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 1G5 (orb2645622) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

alpha catenin

UniProt

P35221