Alpha-adducin (Phospho-T445) Antibody

SKU: orb304677

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Description

The Alpha-adducin (Phospho-T445) Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T445 of human Alpha-adducin protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

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Tested Applications	IHC, WB
Dilution range	WB: 1:500-1000, IHC-P: 1:100-200
Reactivity	Human, Mouse, Porcine, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T445 of human Alpha-adducin protein. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Disclaimer	For research use only

Alternative Names

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ADDA; Alpha-adducin; Erythrocyte adducin subunit alpha

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western blot analysis of Alpha-adducin (Phospho-T445) expression in mouse liver (A), rat liver (B) whole cell lysates. (Predicted band size: 80 kD; Observed band size: 130 kD)

Immunohistochemical analysis of Alpha-adducin (Phospho-T445) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

Q63028, Q9QYC0, P35611

NCBI Gene ID

11518, 118, 24170

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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