alpha Actinin 4 ACTN4 Monoclonal Antibody

SKU: orb547921

Description

Anti-alpha Actinin 4 ACTN4 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB 1:500-2000IHC 1:50-200ICC/IF 1:50-200IP 1:20FC 1:20. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: ACIO-1
• Immunogen: A synthesized peptide derived from human alpha Actinin 4
• Molecular Weight: 45 kDa
• Purification: Affinity-chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

Ubiquitin D; Diubiquitin; Ubiquitin-like protein FAT10; UBD; FAT10

Western blot analysis of ACTN4 using anti-ACTN4 antibody (orb547921) in A431 cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTN4 antigen affinity purified polyclonal antibody (Catalog # orb547921) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system.

Quick Database Links

UniProt

O43707