Alpha-actin-1 Rabbit Polyclonal Antibody

SKU: orb213515

Description

The Alpha-actin-1 Antibody is suitable for IHC, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Alpha-actin-1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
• Reactivity: Bovine, Gallus, Human, Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Alpha-actin-1. The exact sequence is proprietary.
• Target: ACTA1
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ACTA; Actin, alpha skeletal muscle; Alpha-actin-1

Western blot analysis of Alpha-actin-1 expression in A549 (A), SP2/0 (B), rat kidney (C) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)

Immunohistochemical analysis of Alpha-actin-1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunoprecipitation of Alpha-actin-1 from 0.5 mg Mouse Skeletal Muscle tissue lysate, using 5 ug of Anti-Alpha-actin-1 Antibody and 50 ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10 min, Mouse Skeletal Muscle tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 min under agitation. Proteins were eluted by addition of 40 ul SDS loading buffer and incubated for 10 min at 70°C; 10 ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with Anti-Alpha-actin-1 Antibody.

Quick Database Links

Gene Symbol

ACTA1

UniProt

P68133, P68136, P68134

NCBI Gene ID

29437, 11459, 58