Anti-alpha 5 beta 1 Integrin [M200 (Volociximab)]

SKU: orb610778

Description

Recombinant monoclonal antibody to alpha 5 beta 1 Integrin

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation

Images & Validation

• Tested Applications: Blocking, ELISA, FC, IF, IHC
• Reactivity: Human

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: IgG4
• Clone No.: M200 (Volociximab)
• Immunogen: Heterodimeric α5β1Fc fusion protein
• Target: alpha 5 beta 1 Integrin
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Fibronectin receptor; CD49 antigen-like family member E; Integrin alpha-F; VLA-5; CD49e.

Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab) PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (orb348884, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 (orb348884) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb348884, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

alpha 5 beta 1 Integrin

UniProt

P08648