AKAP4 Rabbit Polyclonal Antibody

SKU: orb665912

Description

Rabbit polyclonal antibody to AKAP4

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500-1000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human AKAP4. The exact sequence is proprietary.
• Target: AKAP4
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-AKAP82 antibody, anti-A-kinase anchor protein 4 antibody, anti-AKAP-4 antibody, anti-A-kinase anchor protein 82 kDa antibody, anti-AKAP 82 antibody, anti-hAKAP82 antibody, anti-Major sperm fibrous sheath protein antibody, anti-HI antibody, anti-Protein kinase A-anchoring protein 4 antibody, anti-PRKA4 antibody

Western blot analysis of AKAP4 expression in mouse testis (A), rat testis (B) whole cell lysates. (Predicted band size: 93; 94 kD; Observed band size: 82 kD)

Immunofluorescent analysis of AKAP4 staining in mouse testis. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

AKAP4

UniProt

O35774, Q60662

NCBI Gene ID

79254, 11643