Anti-AIF1 [GT1-mAb2]

SKU: orb2645765

Description

Anti-AIF1 [GT1-mAb2]

Images & Validation

• Tested Applications: ELISA, FC, IF
• Reactivity: Human

Key Properties

• Host: Goat
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: GT1-mAb2
• Immunogen: This antibody was obtained by sequencing a polyclonal goat IgG raised against a peptide with the sequence C-TGPPAKKAISELP.
• Target: AIF1

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

AIF1; IBA1; allograft inflammatory factor 1; AIF-1; IRT-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; Daintain; DADB-70P7.8; allograft inflammatory factor-1 splice variant Hara-1; IRT1; protein G1

Flow cytometry using anti-AIF1 antibody GT1-mAb2. Paraformaldehyde fixed human peripheral blood monocytes permeabilized with 0.5% Triton were stained with the isotype control antibody (black line) or the rabbit IgG version of GT1-mAb2 (orb2645764, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of U937 cells with anti-AIF1 antibody GT1-mAb2. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of GT1-mAb2 (orb2645764) (10 ug/ml) for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645764, DAPI, merged channels and an isotype control. The isotype control was an anti-fluorescein antibody followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

AIF1

UniProt

P55008