ADCK5 Antibody

SKU: orb215399

Description

The ADCK5 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ADCK5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB: 1-500-1-1000, IP: 1-10-1-100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ADCK5. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Uncharacterized aarF domain-containing protein kinase 5

Western blot analysis of ADCK5 expression in Jurkat (A), SW13 (B) whole cell lysates. (Predicted band size: 65 kD; Observed band size: 66 kD)

Immunofluorescent analysis of ADCK5 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

UniProt

Q3MIX3

NCBI Gene ID

203054

References

Darshan S Chandrashekar 1, Farrukh Afaq 2, Santhosh Kumar Karthikeyan 2, Mohammad Athar 3, Sadeep Shrestha 4, Rajesh Singh 5, Upender Manne 6, Sooryanarayana Varambally Bromodomain inhibitor treatment leads to overexpression of multiple kinases in cancer cells Neoplasia, (2024)

PubMed: 39241280

Applications

WB

Reactivity

Human