Actin (muscle) Antibody

SKU: orb44724

Description

Mouse Monoclonal to Actin.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ICC, IHC-Fr, IHC-P, WB
• Reactivity: Canine, Feline, Gallus, Human, Mouse, Primate, Rabbit, Rat
• Application Notes:
  Western blotting: Recommended dilution: 1 μg/ml; positive control: murine femoral muscle, murine heart, negative control: HUVEC line; reducing conditions. Immunohistochemistry (paraffin sections): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: HHF35
• Immunogen: SDS extracted protein fraction of human myocardium
• Target: Actin (muscle)
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Muscle specific actin

Western blotting analysis of muscle-specific actin using the monoclonal antibody HHF35. Lane 1: murine femoral muscle; Lane 2: murine heart; Lane 3: HeLa; Lane 4: HUVEC.

Immunohistochemistry staining of human muscle (paraffin-embedded sections) with anti-muscle-specific actin (HHF35).

Quick Database Links

Gene Symbol

Actin (muscle)

NCBI Gene ID

35