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Description
Research Area
Musculoskeletal & Connective Tissue Research
Images & Validation
−
| Tested Applications | ELISA, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 3.513888889, IHC: 0.388888889, ICC: 1:200-1:500 |
| Reactivity | All |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | C4 |
| Immunogen | Preparation of chicken gizzard actin |
| Target | Actin |
| Molecular Weight | 42 |
| Purification | Mouse ascites fluid |
| Conjugation | Unconjugated |
Customer Validated Data
− The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution
Latest Experiments
9 ResultsDilution
Sample
Summary
WB
Mouse
1:1000
Hippocampal tissue lysates from wild-type and MSK1 null mouse
Sample preparation involves dissecting the hippocampus,...
WB
Mouse
-
Mouse mammary tumor tissues
Sample preparation involves snap-freezing tumor pieces,...
WB
Human
1:5000
Cell lysates from human breast cancer T47D cells
T47D cells culture with specified compounds...
WB
Mouse
1:100000
Suprachiasmatic nucleus (SCN) tissue and piriform cortex tissue lysates
Microdissected SCN and cortical tissues are...
WB
Human
-
Human breast cancer cell lines, MCF-7 and T47D cells
Culture cells in low density/compliant (1.2...
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Ascites fluid + 0.01% NaN3. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−a actin antibody, ACTA antibody, ACTA1 antibody, Actin alpha skeletal muscle antibody, Actin antibody, actin alpha 1 skeletal muscle 1 antibody, actin alpha 1 skeletal muscle antibody, Actin alpha skeletal muscle antibody, actina antibody, actine antibody, ACTS_HUMAN antibody, aktin antibody, Alpha Actin 1 antibody, Alpha skeletal muscle Actin antibody, alpha skeletal muscle antibody, alpha-actin antibody, Alpha-actin-1 antibody, ASMA antibody, CFTD antibody, CFTD1 antibody, CFTDM antibody, MPFD antibody, NEM1 antibody, NEM2 antibody, NEM3 antibody, nemaline myopathy type 3 antibody
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Quick Database Links
Gene Symbol
Actin
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Actin Antibody (orb319459)
- 0.0
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