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Anti-ACOX1 Antibody

Catalog Number: orb341430

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb341430
CategoryAntibodies
DescriptionRabbit polyclonal antibody to ACOX1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human ACOX1
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1:2000, IHC-P: 1:50-1:200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetACOX1
Entrez51, 11430
UniProt IDQ15067, Q9R0H0
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesACOX; Peroxisomal acyl-coenzyme A oxidase 1; AOX;
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-ACOX1 Antibody

Western blot analysis of ACOX1 expression in NCIH460 (A), mouse liver (B), mouse kidney (C) whole cell lysates. (Predicted band size: 70; 74 kD; Observed band size: 74; 50 kD)

Anti-ACOX1 Antibody

Immunohistochemical analysis of ACOX1 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-ACOX1 Antibody

Immunofluorescent analysis of ACOX1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    IF,  IH,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    200 μl, 100 μl, 50 μl
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    IF,  IP,  WB

    Human, Mouse, Rat

    Rabbit

    Monoclonal

    Unconjugated

    200 μl, 100 μl, 30 μl
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    ELISA,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
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    ELISA,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
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    ELISA,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    iFluor647

    100 μg