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Anti-ACOX1 Antibody

Catalog Number: orb1473774

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1473774
CategoryAntibodies
DescriptionMouse monoclonal antibody to ACOX1
Species/HostMouse
ClonalityMonoclonal
Tested applicationsIF, IH, WB
ReactivityHuman
ImmunogenRecombinant fusion protein of human ACOX1. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50)
Form/AppearanceMouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetACOX1
Entrez51
UniProt IDQ15067
SourceMouse
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesMouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesACOX; Peroxisomal acyl-coenzyme A oxidase 1; AOX;
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-ACOX1 Antibody

Western blot analysis of ACOX1 expression in K562 (A) whole cell lysates. (Predicted band size: 74 kD; Observed band size: 74 kD)

Anti-ACOX1 Antibody

Immunohistochemical analysis of ACOX1 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-ACOX1 Antibody

Immunofluorescent analysis of ACOX1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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