Anti-ABI1 Antibody

• Catalog Number: orb214949
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ABI1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ABI1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ABI1
• Entrez: 10006, 79249, 11308
• UniProt ID: Q8CBW3, Q8IZP0, Q9QZM5
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti SSH3BP1 antibody, anti Abl interactor 1 antib
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ABI1 expression in rat brain (A), U87MG (B), Myla2059 (C), K562 (D) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 65 kD)

Immunofluorescent analysis of ABI1 staining in VB2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).