ABHD5 Rabbit Monoclonal Antibody

SKU: orb548172

Description

ABHD5 Rabbit Monoclonal Antibody

Research Area

Cancer Biology, Cardiovascular Research, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution Range: WB 1:500-2000 IHC 1:50-200 FC 1:100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: AFHE-1
• Immunogen: A synthesized peptide derived from human ABHD5 Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.
• Target: 1-acylglycerol-3-phosphate O-acyltransferase ABHD5
• Molecular Weight: 34 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glutamine synthetase; GS; 6.3.1.2; Glutamate decarboxylase; 4.1.1.15; Glutamate--ammonia ligase; GLUL; GLNS

Western blot analysis of ABHD5 expression in HepG2 cell lysate using anti-ABHD5 antibody (orb548172). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABHD5 antigen affinity purified polyclonal antibody (Catalog # orb548172) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for ABHD5.

Quick Database Links

Gene Symbol

1-acylglycerol-3-phosphate O-acyltransferase ABHD5

UniProt

Q8WTS1