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Anti-ABHD5 Monoclonal Antibody
Catalog Number: orb548172
| Catalog Number | orb548172 |
|---|---|
| Category | Antibodies |
| Description | Anti-ABHD5 Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Monoclonal |
| Clone Number | AFHE-1 |
| Tested applications | FC, IHC, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Immunogen | A synthesized peptide derived from human ABHD5 Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. |
| Antibody Type | Primary Antibody |
| Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 34 kDa |
| UniProt ID | Q8WTS1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Glutamine synthetase; GS; 6.3.1.2; Glutamate decar Read more... |
| Note | For research use only |
| Application notes | WB 1:500-1:2000IHC 1:100-1:500FC 1:100. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of ABHD5 expression in HepG2 cell lysate using anti-ABHD5 antibody (orb548172). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABHD5 antigen affinity purified polyclonal antibody (Catalog # orb548172) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for ABHD5.
