5-HT5A Rabbit Polyclonal Antibody

SKU: orb665811

Description

The 5-HT5A Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human 5-HT5A. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, GPCR, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500-1000, IF: 1-100-500
• Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human 5-HT5A. The exact sequence is proprietary.
• Target: HTR5A
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

5-hydroxytryptamine receptor 5A; 5-HT-5; 5-HT-5A; 5-HT5A; Serotonin receptor 5A

Western blot analysis of 5-HT5A expression in rat kidney (A) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 40 kD)

Immunofluorescent analysis of 5-HT5A staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

HTR5A

UniProt

P35364, P47898

NCBI Gene ID

25689, 3361

Yu-Long Zhao 1, Jia-Liang Xu 1, Hui-Yuan Yi 1, Sani Sa'idu Baba 2, Yi-Xiao Guo 1, Xue-Mei Hou 1, Xiao-Cui Yuan 1, Xu-Hui Li 3, Yu-Ying Wang 1, Ling-Li Liang 1, Fu-Quan Huo Activation of 5-HT5A receptor in the ventrolateral orbital cortex produces antinociceptive effects in rat models of neuropathic and inflammatory pain Neuropharmacology, (2024)

PubMed: 38160874

Applications

IF

Reactivity

Rat