Anti-4-1BB [4B4-1-1]

• Catalog Number: orb758986
• Category: Antibodies
• Description: Rabbit monoclonal antibody to 4-1BB
• Target: 4-1BB
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: The original antibody was generated by immunizing BALB/c mice with a fusion protein of human 4-1BB antigen and glutathione s transferase (GST).
• UniProt ID: Q07011
• Tested applications: Blocking, ELISA, FC, IHC
• Clone Number: 4B4-1-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD137; MGC2172; Tumor necrosis factor receptor sup
• Note: For research use only

Immunofluorescence staining of fixed Jurkat cells with anti-4-1BB antibody 4B4-1-1. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 4B4-1-1 (orb758986) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758986, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-4-1BB antibody 4B4-1-1 Human thymus(A), lymph node(B) and tonsil(C) tissue lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 4B4-1-1 (orb758986) at 0.3 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.