Anti-2B4 antibody(DM69); Rabbit mAb

• Catalog Number: orb757392
• Category: Antibodies
• Description: Anti-2B4 antibody(DM69), Rabbit mAb
• Target: 2B4
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: Q9BZW8
• Tested applications: ELISA, FC
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DM69
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD244,2B4,SLAMF4,NKR2B4,NAIL,h2B4
• Note: For research use only

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human 2B4 protein, mFc-His tagged protein orb689371 can bind Rabbit anti-2B4 monoclonal antibody (clone: DM69) in a linear range of 1-100 ng/ml.

Expi 293 cell line transfected with irrelevant protein (A) and human 2B4 (B) were surface stained with Rabbit anti-2B4 monoclonal antibody 1 μg/ml (clone: DM69) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry data of serially titrated Rabbit anti-2B4 monoclonal antibody (clone: DM69) on THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Affinity ranking of different Rabbit anti-2B4 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.