ANO5 Antibody (C-term)

• Catalog Number: orb1928420
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB22028
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 107188 Da
• Target: This ANO5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 792-819 amino acids from the C-terminal region of human ANO5.
• UniProt ID: Q75V66
• NCBI: NP_998764.1, NP_001136121.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anoctamin-5, Gnathodiaphyseal dysplasia 1 protein,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ANO5 Antibody (C-term) in mouse spleen tissue lysates (35 ug/lane). ANO5 (arrow) was detected using the purified Pab.

All lanes: Anti-ANO5 Antibody (C-term) at 1:1000 dilution. Lane 1: human brain lysate. Lane 2: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 107 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

ANO5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of theANO5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.