Annexin V Antibody (N-term)

SKU: orb1929970

Description

Annexin V Antibody (N-term)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: IHC-P-Leica - 1:500, WB - 1:2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Monkey

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 35937 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ANX5, ENX2, PP4

Annexin V Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-Annexin V Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: 293 whole cell lysate. Lane 3: U-2OS whole cell lysate. Lane 4: A2058 whole cell lysate. Lane 5: T47D whole cell lysate. Lane 6: Mouse lung lysate. Lane 7: Mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P08758

Senjuti Dasgupta et al. Clinicopathological Study of Placentae of Twin Pregnancies with Emphasis on Exploration of Expression of Two Novel Markers, Annexin A5 and Apelin Turk Patoloji Derg, (2026)

PubMed: 41968879

Applications

IHC

Reactivity

Human