Annexin V Antibody (N-term)

• Catalog Number: orb1929970
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19461
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 35937 Da
• Target: This Annexin V antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Annexin V.
• UniProt ID: P08758
• NCBI: NP_001145.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Annexin A5, Anchorin CII, Annexin V, Annexin-5, Ca
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Annexin V Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-Annexin V Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: 293 whole cell lysate. Lane 3: U-2OS whole cell lysate. Lane 4: A2058 whole cell lysate. Lane 5: T47D whole cell lysate. Lane 6: Mouse lung lysate. Lane 7: Mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.