Annexin V Antibody (N-term)

SKU: orb1929970

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P-Leica - 1:500, FC - 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Monkey

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD31683
• Immunogen: This Annexin V antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Annexin V. Antigen Region: 1-30 aa.
• Target: ANXA5
• Molecular Weight: 35937 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, CBP-I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, PP4, Placental anticoagulant protein I, PAP-I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, VAC-alpha, ANXA5, ANX5, ENX2, PP4

Annexin V Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-Annexin V Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: 293 whole cell lysate. Lane 3: U-2OS whole cell lysate. Lane 4: A2058 whole cell lysate. Lane 5: T47D whole cell lysate. Lane 6: Mouse lung lysate. Lane 7: Mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

ANXA5

UniProt

P08758

RefSeq (Protein):NP_001145.1

Senjuti Dasgupta et al. Clinicopathological Study of Placentae of Twin Pregnancies with Emphasis on Exploration of Expression of Two Novel Markers, Annexin A5 and Apelin Turk Patoloji Derg, (2026)

PubMed: 41968879

Applications

IHC

Reactivity

Human

Senjuti Dasgupta a , Uma Banerjee a , Partha Mukhopadhyay b , Biplab Das a , Saswata Saha Placental expression of Annexin A5, Apelin, and Caspase-3 in gestational hypothyroidism: A clinicopathological study European Journal of Obstetrics & Gynecology and Reproductive Biology, (2025)

PubMed:

Applications

IHC

Reactivity

Human