Ankyrin R Antibody

SKU: orb376692

Description

Mouse Anti-Human Ankyrin R Monoclonal IgG2B

Research Area

Cardiovascular Research, Cell Biology, Disease Biomarkers, Hematology, Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:100 dilution was sufficient for detection of Ankyrin R in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone No.: N388A/10 (Formerly sold as S388A-10)
• Immunogen: Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R
• Target: Ankyrin R
• Molecular Weight: 200kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.1% sodium azide. Storage buffer changes when conjugated.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ankyrin R, Ankyrin-R, Ankyrin-1, Ankyrin 1, ANK1, ANK-1, Ankyrin, Erythrocyte ankyrin, rCG_43073

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin R Monoclonal Antibody, Clone N388A/10. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin R Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin R Antibody (D) Composite.

Western Blot analysis of Rat Brain showing detection of ~200 kDa Ankyrin-R protein using Mouse Anti-Ankyrin-R Monoclonal Antibody, Clone N388A/10. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 μg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Ankyrin-R Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.

Quick Database Links

Gene Symbol

Ankyrin R

UniProt

P16157

NCBI Gene ID

286

RefSeq (Protein):NP_000028.3