Ankyrin G Antibody (HRP)

SKU: orb149897

Description

Mouse monoclonal to Ankyrin G (HRP). Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton. Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane..

Research Area

Cell Biology, Disease Biomarkers, Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of Ankyrin-G in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N106/20 (Formerly sold as S106-20)
• Immunogen: Fusion protein 1000 C-terminal amino acids of human Ankyrin G (also known as ANK-3 or ankyrin-3) encompassing all of Ankyrin G with the exception of Ankyrin repeats
• Target: Ankyrin G
• Molecular Weight: 200kDa
• Purification: Protein G Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ankyrin G, Ankyrin-G, Ankyrin-3, Ankyrin 3, ANK3, ANK-3, Ankyrin 3 (G), Ankyrin G119, Ankryin G119, Brain-specific ankyrin G, CHANK3, Node of Ranvier (ankyrin G), Node of Ranvier, FLJ45464, OTTHUMP00000217458, OTTHUMP00000217575, RP11-369L1.1

Quick Database Links

Gene Symbol

Ankyrin G

UniProt

Q12955

NCBI Gene ID

288

RefSeq (Protein):NP_066267.2