Ankyrin B Antibody (FITC)

SKU: orb149828

Description

Mouse monoclonal to Ankyrin B (FITC). Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton. Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane..

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution range: WB (1:1000), IHC (1:200), ICC/IF (1:200)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-400 was sufficient for detection of Ankyrin-B in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N105/13 (Formerly sold as S105-13)
• Immunogen: Synthetic peptide amino acids 203-496 of human Ankyrin-B
• Target: Ankyrin B
• Molecular Weight: 200kDa
• Purification: Protein G Purified
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Ankyrin B, Ankyrin-B, Ankyrin-2, Ankyrin 2, ANK2, ANK-2, Ankyrin nonerythroid, non-erythroid ankyrin, Brain ankyrin

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin B Monoclonal Antibody, Clone N105/13. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin B Antibody (D) Composite.

Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ankyrin B Antibody. (D) Composite.

Quick Database Links

Gene Symbol

Ankyrin B

UniProt

Q01484

NCBI Gene ID

287

RefSeq (Protein):NP_001120965.1