ANKK1 Antibody

SKU: orb1246447

Description

ANKK1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx. 85kDa band observed in Human Brain (Substantia nigra and Amygdala) lysates (calculated MW of 84.6 kDa according to NP_848605.1). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5-10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SRQADPNLHEAEGKT
• Target: ANKK1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ANKK1, ankyrin repeat and kinase domain containing 1, PKK2, X-kinase, protein kinase PKK2

orb1246447 (0.1 ug/ml) staining of Human Substantia Nigra lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246447 (5 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ANKK1

UniProt

Q8NFD2

RefSeq (Protein):NP_848605.1