ANKH Antibody (C-term)

• Catalog Number: orb1927755
• Category: Antibodies
• Description: ANKH Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q9HCJ1
• MW: 54241 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB - 1:2000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: KIAA1581
• Research Area: Signal Transduction
• Note: For research use only

Western blot analysis of ANKH Antibody (C-term) in mouse cerebellum tissue lysates (35 ug/lane). ANKH (arrow) was detected using the purified Pab.

ANKH Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-ANKH Antibody (C-term) at 1:1000 dilution + U-2OS whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

ANKH Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANKH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.