Angiopoietin 4 Polyclonal Antibody

• Catalog Number: orb1421318
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Angiopoietin 4.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Porcine, Rat
• UniProt ID: Q9Y264
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Angiopoietin-4, ANG-4, Angiopoietin-3, ANG-3, ANGP
• Note: For research use only

Sample: Lung (Mouse) Lysate at 30 ug. Lung (Rat) Lysate at 30 ug. Primary: Anti- Anti-ANGPT3/ANG3/ANGPT4/ANG4 at 1/400 dilution. Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/3000 dilution. Predicted band size: 57kD. Observed band size: 57kD.

Tissue/cell: mouse tumor tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ANGPT3/ANG3/ANGPT4/ANG4 Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.