ANAPC5 (vPairTM) Antibodies

SKU: orb131841

Description

Rabbit polyclonal to ANAPC5. ANAPC5 antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Biorbyt to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody pairs-based assays. The use of a pair of fully characterized vPair antibodies in the same assay can validate signal specificity since vPair antibodies recognize two independent epitopes of the same protein. Different sets of vPair antibodies are developed at Biorbyt to work with specific applications, including antibody pairs arrays, Western blot, IP-Western, ChIP, IHC, and FACS. Anaphase-promoting complex subunit 5 (ANAPC5) belongs to the APC5 family and is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of Lys-11-linked polyubiquitin chains and, to a lower extent, the formation of Lys-48-and Lys-63-linked polyubiquitin chains.

Research Area

Epigenetics

Images & Validation

• Tested Applications: WB
• Dilution Range: WB:1:1000-1:3000
• Reactivity: Human, Mouse
• Application Notes:
  WB: The apparent protein size on WB may be different from the calculated M.W. due to modifications.; ; Experiment Notes: Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal or the C-terminal region of human ANAPC5.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: N-terminal or the C-terminal region of human ANAPC5
• Molecular Weight: 85
• Purity: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01% NaN3
• Concentration: 100 μg/ml BSA
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Anaphase-promoting complex subunit 5 antibody pairs, anti APC5 antibody pairs, anti Cyclosome subunit 5 antibody pairs

Quick Database Links

UniProt

Q9UJX4