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ANAPC5 Antibody

Catalog Number: orb1256589

DispatchUsually dispatched within 5-10 working days
$ 960.00
Catalog Numberorb1256589
CategoryAntibodies
DescriptionANAPC5 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIHC, IP, WB
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2).
Antibody TypePrimary Antibody
Concentrationbatch dependent
Form/AppearanceLiquid
ConjugationUnconjugated
MWObserved: 85kDa
TargetANAPC5
UniProt IDQ9UJX4
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid
Alternative namesAPC5 Antibody: APC5, APC5, Anaphase-promoting comp
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NoteFor research use only
Application notesWB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
Expiration Date12 months from date of receipt.
ANAPC5 Antibody

Western blot analysis of extracts of various cell lines, using ANAPC5 antibody (orb1256589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

ANAPC5 Antibody

Immunohistochemistry of paraffin-embedded rat kidney using ANAPC5 antibody (orb1256589) at dilution of 1:100 (40x lens).

ANAPC5 Antibody

Immunohistochemistry of paraffin-embedded human kidney using ANAPC5 antibody (orb1256589) at dilution of 1:100 (40x lens).

ANAPC5 Antibody

Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells, using 3 ug ANAPC5 antibody (orb1256589). Western blot was performed from the immunoprecipitate using ANAPC5 antibody (orb1256589) at a dilution of 1:1000.

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