ANAPC5 Antibody

• Catalog Number: orb1256589
• Category: Antibodies
• Description: ANAPC5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 85kDa
• Target: ANAPC5
• UniProt ID: Q9UJX4
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: APC5 Antibody: APC5, APC5, Anaphase-promoting comp
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ANAPC5 antibody (orb1256589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using ANAPC5 antibody (orb1256589) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney using ANAPC5 antibody (orb1256589) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells, using 3 ug ANAPC5 antibody (orb1256589). Western blot was performed from the immunoprecipitate using ANAPC5 antibody (orb1256589) at a dilution of 1:1000.