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Catalog Number | orb1256589 |
---|---|
Category | Antibodies |
Description | ANAPC5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 85kDa |
Target | ANAPC5 |
UniProt ID | Q9UJX4 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | APC5 Antibody: APC5, APC5, Anaphase-promoting comp Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using ANAPC5 antibody (orb1256589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using ANAPC5 antibody (orb1256589) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using ANAPC5 antibody (orb1256589) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells, using 3 ug ANAPC5 antibody (orb1256589). Western blot was performed from the immunoprecipitate using ANAPC5 antibody (orb1256589) at a dilution of 1:1000.
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |