ANAPC2 Antibody (C-term)

SKU: orb306322

Description

Rabbit polyclonal antibody to ANAPC2

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:25, WB - 1:1000, IHC-P - 1:25
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD73898
• Immunogen: This ANAPC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 712-746 amino acids from the C-terminal region of human ANAPC2. Antigen Region: 712-746 aa.
• Target: ANAPC2
• Molecular Weight: 93828
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Anaphase-promoting complex subunit 2 antibody, anti APC2 antibody, anti Cyclosome subunit 2 antibody, anti ANAPC2 antibody, anti APC2 antibody, anti KIAA1406 antibody

Western blot analysis of lysate from human testis tissue lysate, using ANAPC2 Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Immunohistochemical analysis of paraffin-embedded H. thymus section using ANAPC2 Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling ANAPC2 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin at 1/100 dilution (red).

Quick Database Links

Gene Symbol

ANAPC2

UniProt

Q9UJX6