ANA-12

SKU: orb1305599

Description

ANA-12 is a selective antagonist of the TrkB receptor. It has demonstrated anxiolytic and antidepressant effects in murine models, making it a valuable research tool for studying neurotrophin signaling in psychiatric disorders and neurobiology.

Research Area

Neuroscience

Key Properties

• CAS Number: 219766-25-3
• MW: 407.49
• Purity: >98%
• Formula: C₂₂H₂₁N₃O₃S
• SMILES: O=C(Nc1ccccc1C(=O)NC1CCCCNC1=O)c1cc2ccccc2s1
• Target: Kinase
• Solubility: Soluble in DMSO (up to 20 mg/ml).

Bioactivity

• Target IC50:
  TrkB:10 nM(Kd)
• In Vivo:
  In male Sprague-Dawley rats, ANA-12 (3 μg/dose) inhibits the medial nucleus of the solitary tract (mNTS) BDNF's effect on reducing food intake. In male C57BL/6 mice, ANA-12 (0.5 mg/kg, intraperitoneally) demonstrates antidepressant effects against lipopolysaccharide-induced depressive-like behavior. In wild-type male mice, ANA-12 reverses ethanol consumption and induces D3 receptor downregulation; however, it is ineffective in D3R -/- mice.
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• In Vitro:
  In nnr5 PC12-TrkB cells, ANA-12 inhibits neurite outgrowth induced by brain-derived neurotrophic factor (BDNF) at concentrations as low as 10 nM. It selectively binds directly to the TrkB receptor, suppressing the downstream TrkB pathway without altering the functions of TrkA and TrkC. Furthermore, ANA-12 nullifies the effect of BDNF on increasing the inward current in DRG neurons.
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• Cell Research:
  Modulation of neurite outgrowth by molecules is assessed in nnr5 PC12–TrkB, –TrkA, and –TrkC cells after addition of BDNF (1 nM), NGF (2 nM), and NT-3 (10 nM), respectively. The number of cells bearing neurites longer than 2 cells in diameter in each counting field is microscopically determined (2 fields per well, 3 wells per condition). Counting is performed blind each 24 hours for 3 days. (Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Etacrynic acid

Compound Identifiers

PubChem CID

2799722