β-Amyloid (25-35)

SKU: orb1308652

Description

The β-Amyloid (25-35) fragment is a neurotoxic peptide derived from the full-length amyloid beta protein. It is widely utilized in both in vitro and in vivo research to model Alzheimer's disease pathology and study associated neurodegenerative mechanisms.

Research Area

Neuroscience

Key Properties

• CAS Number: 131602-53-4
• MW: 1060.27
• Purity: >99.99% (May vary between batches)
• Formula: C₄₅H₈₁N₁₃O₁₄S
• SMILES: CCC(C)C(NC(=O)C(NC(=O)C(C)NC(=O)CNC(=O)C(CCCCN)NC(=O)C(CC(N)=O)NC(=O)C(CO)NC(=O)CN)C(C)CC)C(=O)NCC(=O)NC(CC(C)C)C(=O)NC(CCSC)C(O)=O
• Target: Beta Amyloid
• Solubility: 10% DMSO+90% Corn Oil:3.3 mg/mL (3.11 mM);DMSO:97 mg/mL (91.49 mM);H2O:3.33 mg/mL (3.14 mM)

Bioactivity

• In Vivo:
  The Alzheimer' s disease (AD) model group present with spatial learning and memory impairments. CDS and donepezil administration significantly ameliorate the Aβ25–35 peptide-induced memory impairment in both Morris water maze (P<0.05) and passive avoidance task (P<0.01) compared to the AD model group. Rats are injected with β-Amyloid (25-35) intracerebroventricularly and compound Danshen (CDS) are subsequently administered once daily for 23 days. Rats' behavior is monitored using Morris water maze and passive avoidance. Real time PCR and Western blotting are used in determining amyloid precursor protein (APP), β-site APP cleaved enzyme-1(BACE1), Presenilin-1 (PS1), Insulin-degrading enzyme (IDE) and neprilysin (NEP) in hippocampus.
• In Vitro:
  The amino acid sequence of β-Amyloid (25-35) is NH2-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH, where the first Gly represents the amino acid 25 and the last Met represents the amino acid 35 and it is also investigated in gel state for the first time. Amyloid beta-peptide(25-35) induces apoptotic effects on isolated brain mitochondria and the redox state of methionine-35, plays a key role in the induction of programmed cellular death pathways and toxic events. Comparative studies are also carried out using vibrational absorption and ECD. The conformational preference of Aβ(25-35) peptide film is also investigated using vibrational absorption and VCD spectroscopy.

Storage & Handling

• Storage: keep away from moisture | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

β-Amyloid, βAmyloid (2535), βAmyloid, β-Amyloid peptide(25-35), β-Amyloid peptide, β-amyloid peptide, β Amyloid (25 35), β Amyloid, inhibit, Inhibitor, b-Amyloid peptide, bAmyloid, b-Amyloid, Aβ25-35, b Amyloid, Amyloid beta-peptide, Amyloid b-peptide, Amyloid beta-peptide(25-35), Amyloid-β, Amyloid β-peptide, Ab25-35, Abeta, Abeta25-35, Beta Amyloid, beta-Amyloid, BetaAmyloid, beta-Amyloid peptide

Compound Identifiers

PubChem CID

3407255