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Catalog Number | orb1938784 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB23658 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 57710 Da |
Target | This AMY2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-100 amino acids from the N-terminal region of human AMY2B. |
UniProt ID | P19961 |
NCBI | NP_066188.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Alpha-amylase 2B,4-alpha-D-glucan glucanohydrolase Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
AMY2B Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AMY2B antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMY2B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
AMY2B Antibody (N-term) western blot analysis in HL-60, CEM and K562 cell line lysates (35 ug/lane). This demonstrates the AMY2B antibody detected the AMY2B protein (arrow).
Confocal immunofluorescent analysis of AMY2B Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).