AMPK-IN-3

SKU: orb2900782

Description

AMPK-IN-3 (compound 67) is a potent and selective AMPK inhibitor with IC50s of 60.7, 107 and 3820 nM for AMPK (α2), AMPK (α1) and KDR, respectively. AMPK-IN-3 inhibits AMPK does not affect cell viability or cause significant cytotoxicity in K562 cells. AMPK-IN-3 can be used in study of cancer.

Key Properties

• CAS Number: 2417674-27-0
• MW: 451.56
• Purity: >98% (HPLC)
• Formula: C₂₅H₃₃N₅O₃
• SMILES: CCN(CC)CCNC(=O)c1c(C)[nH]c(\C=C2/C(=O)Nc3ccc(CCC(N)=O)cc23)c1C
• Target: VEGFR
• Solubility: In Vitro: DMSO : 115 mg/mL (254.67 mM; Ultrasonic )

Bioactivity

• In Vitro:
  AMPK-IN-3 (100 nM) shows inhibition values for AMPK(α2), FLT1, JAK1 JH2-pseudokinase and AMPK(α1) for 64%, 43%, 41% and 29%, respectively. AMPK-IN-3 (0.195313, 0.78125, 3.125, 12.5, 50 μM; 2 h) decreases the level of p-ACC in K562 cells. AMPK-IN-3 (1-100 μM; 24, 48, 72 h) shows potent inhibition of cellular AMPK activity but not affect cell viability. Cell Viability Assay Cell line: K562 cells. Concentration: 0.195313, 0.78125, 3.125, 12.5, 50 μM Incubation time: 2 h. Result: Decreased cellular levels of p-ACC(Ser79) in K562 cells. Cell Viability Assay Cell line: K562 cells. Concentration: 1-100 μM. Incubation time: 24, 48, 72 h. Result: Showed no measurable impact on cell viability in K562 cells cultured under hypoxic conditions for 72 hours.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

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Gene Symbol

VEGFR