AMPK gamma1 Mouse Monoclonal Antibody

SKU: orb526514

Description

AMPK gamma1 Mouse Monoclonal Antibody

Research Area

Cancer Research, Cell Biology, Cholesterol Metabolism, Energy Metabolism, Fatty Acid Oxidation, Lipid Metabolism, Metabolism, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 5H5
• Immunogen: Recombinant human AMPK gamma1 Protein (118-206/331aa)
• Target: PRKAG1
• Molecular Weight: 36 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRKAG 1; PRKAG1; 5-AMP-activated protein kinase gamma-1 subunit; 5' AMP activated protein kinase subunit gamma 1; AMP activated protein kinase noncatalytic gamma 1 subunit; AMPK gamma 1 chain; AMPKg; MGC8666; PRKAG 1; PRKAG1; Protein kinase AMP-activated gamma 1 non-catalytic subunit.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AMPK gamma1) Monoclonal Antibody, Unconjugated (orb526514) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AMPK gamma1) Monoclonal Antibody, Unconjugated (orb526514) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Quick Database Links

Gene Symbol

PRKAG1

UniProt

P54619