AMPK beta 1 (1A7) Mouse mAb

SKU: orb1563338

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Description

Research Area

Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	ICC, IF, IHC-Fr, IHC-P, IP, WB
Dilution Range	WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Reactivity	Human, Monkey, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	1A7-E11-E9
Immunogen	Purified recombinant human AMPK beta 1 protein fragments expressed in E.coli.
Target	PRKAB1
Molecular Weight	Calculated MW: 30 kDa; Observed MW: 38 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
Concentration	2.47 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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5' ' -AMP-activated protein kinase subunit beta-1; AMP-activated; noncatalytic; beta-1; AMPK; AMPK beta 1 chain; AMPK subunit beta-1; AMPK-BETA-1; AMPKb; HAMPKb; PRKAB1

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Immunoprecipitation analysis of AMPK beta 1 (1A7) in Hela lysates using AMPK beta 1 antibody.

Western blot analysis of AMPK beta 1 in 3T3, Hela, PC-12, COS7 and MDA-MB-468 lysates using AMPK beta 1 antibody.

Immunocytochemistry analysis of AMPK beta 1 (1A7) in HeLa using AMPK beta 1 antibody.

Immunohistochemistry analysis of paraffin-embedded Breast cancer using AMPK beta 1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.