AMPK alpha (PRKAA1) Antibody (S487)

• Catalog Number: orb1939489
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB13341
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 64009 Da
• Target: This AMPK alpha(PRKAA1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-502 amino acids from human AMPK alpha(PRKAA1).
• UniProt ID: Q13131
• NCBI: NP_006242.5, NP_996790.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: 5'-AMP-activated protein kinase catalytic subunit
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-PRKAA1 Antibody (S487) at 1:2000 dilution + K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 64 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PRKAA1-pS487, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of PRKAA1 (arrow) using rabbit polyclonal PRKAA1 Antibody (S246). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA1 gene (Lane 2).