AMPK alpha 1 (Phospho-Ser496) Rabbit mAb [KD Validated] Antibody

SKU: orb1727738

Description

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS.

Images & Validation

• Tested Applications: ICC, IP, WB
• Dilution Range: WB:1:1000~1:2000ICC/IF:1:50~1:200IP:1:50
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human AMPK alpha 1
• Molecular Weight: 64kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

5'-AMP-activated protein kinase catalytic subunit alpha-1; AAPK1; AMP-activate kinase alpha 1 subunit; AMP-activated protein kinase; AMPK; AMPK alpha 1; AMPK subunit alpha-1; PRKAA 1; ACACA kinase;

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of Phospho-AMPK alpha 1 (S496) expression in (1) 293T cell lysate treated with LP; (2) 293T cell lysate.

Quick Database Links

UniProt

Q13131