Aminoallyl-dUTP-ATTO-680

• Catalog Number: orb1733883
• Category: Tools
• Description: Aminoallyl-dUTP-ATTO680 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-ATTO680/dTTP ratio for PCR and Nick Translation: 30% Aminoallyl-dUTP-680/70% dTTP.
• Concentration: 1.0 mM-1.1 mM
• Form/Appearance: solution in water; Color: blue; pH: 7.5 ± 0.5
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 1030.83 g/mol (free acid); Detected MW: 1030.20 g/mol (free acid)
• SMILES: [C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCN1c2c(C(=CC1(C)C)CS(=O)(=O)[O-])cc1c(c2)Oc2c(=N1)cc1c(=[N+](CCC1)CC)c2)O
• Formula: C₃₉H₄₉N₆O₁₉P₃S
• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Note: For research use only
• Application notes: Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. ; Spectroscopic Propertie: λexc 681 nm, λem 698 nm, ε 125.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
• Expiration Date: 12 months from date of receipt.