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Catalog Number | orb64150 |
---|---|
Category | Tools |
Description | Aminoallyl-dUTP-ATTO680 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-ATTO680/dTTP ratio for PCR and Nick Translation: 30% Aminoallyl-dUTP-680/70% dTTP. |
Concentration | 1.0 mM-1.1 mM |
Form/Appearance | solution in water; Color: blue; pH: 7.5 ± 0.5 |
Purity | ≥ 95% (HPLC) |
MW | Theoretical MW: 1030.83 g/mol (free acid); Detected MW: 1030.20 g/mol (free acid) |
SMILES | [C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCN1c2c(C(=CC1(C)C)CS(=O)(=O)[O-])cc1c(c2)Oc2c(=N1)cc1c(=[N+](CCC1)CC)c2)O |
Formula | C39H49N6O19P3S |
Storage | store at -20 °C |
Note | For research use only |
Application notes | Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. < br>< b>Spectroscopic Propertie: λexc 681 nm, λem 698 nm, ε 125.0 L mmol-1 cm-1 (Tris-HCl pH 7.5). |
Expiration Date | 12 months from date of receipt. |