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Description
Images & Validation
−| Tested Applications | ChIP, ELISA, EM, FC, ICC, IHC-P, IP, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try:IHC-P: Use a concentration of 1 µg/ml.ICC/IF: 1/50. PubMed: 17103222WB: 1/1500. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa). |
| Reactivity | Human, Xenopus |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 11G5 |
| Immunogen | Full length recombinant human ALY/Ref fusion protein |
| Target | ALY/Ref |
| Purification | Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody (from supernatant) containing PBS 0.1% sodium azide |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Ally of AML1 and LEF1 antibody;BEF antibody;bZIP enhancing factor antibody;REF antibody;THO complex 4antibody;THOC 4 antibody;THOC-4 antibody;THOC4 antibody;Transcriptional coactivatorantibody
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
ChIP
Chromatin Immunoprecipitation
EM
Electron Microscopy





