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| Catalog Number | orb197847 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to ALPI |
| Target | ALPI |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Concentration | 1mg/ml |
| Buffer/Preservatives | Ascitic fluid containing 0.03% sodium azide. |
| Immunogen | Purified recombinant fragment of human ALPI (AA: 397-458) expressed in E. Coli. |
| UniProt ID | P09923 |
| MW | 56.8kDa |
| Tested applications | ELISA, FC, WB |
| Dilution range | WB: 1/500 - 1/2000, FC: 1/200 - 1/400, ELISA 1/10000 |
| Antibody Type | Primary Antibody |
| Clone Number | 3B10E7 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | IAP |
| Background | There are at least four distinct but related alkal Read more... |
| Note | For research use only |
| Entrez | 248 |

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of ALPI antibody

Flow cytometric analysis of HepG2 cells using ALPI mouse mAb (green) and negative control (purple)

Western blot analysis using ALPI mAb against human ALPI recombinant protein. (Expected MW is 31.9 kDa)

Western blot analysis using ALPI mAb against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate

Figure 3: Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.
Rat | |
0.79-50 ng/mL | |
0.33 ng/mL |
Human | |
1.57-100 ng/mL | |
0.56 ng/mL |
Animal | |
1.57-100 ng/mL | |
0.53 ng/mL |
IF, IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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